CompoundSophoraeDecoction:treatingulcerativecolitisbyaffectingmultiplemetabolicpathways.

复方苦参汤通过影响多种代谢途径治疗UC

Abstract

Ulcerativecolitis(UC)isachronicrefractorynon-specificintestinalinflammatorydiseasethatisdifficulttobecured.Thediscoveryofnewulcerativecolitis-relatedmetabolitebiomarkersmayhelpfurtherunderstandUCandfacilitateearlydiagnosis.ItmayalsoprovideabasisforexplainingthemechanismofdrugactioninthetreatmentofUC.CompoundSophoraeDecoction(CSD)isanempiricalformulausedintheclinicaltreatmentofUC.Althoughitisknowntobeefficacious,itsmechanismofactioninthetreatmentofUCisunclear.ThepurposeofthisstudywastoinvestigatethechangesinendogenoussubstancesinUCratsandtheeffectsofCSDonmetabolicpathwaysusingthemetabonomicsapproach.MetabolomicsstudiesinratswithUCandnormalratswereperformedusingLC-MS/MS.RatswithUCinducedusingTNBSenemawereusedasthestudymodels.Metabolicprofilingandpathwayanalysisofbiomarkerswasperformedusingstatisticalandpathwayenrichmentanalyses.36screenedpotentialbiomarkerswerefoundtobesignificantlydifferentbetweentheUCandthenormalgroups;itwasalsofoundthatCSDcouldmodulatethelevelsofthesepotentialbiomarkers.CSDwasfoundtobeefficaciousinUCbyregulatingmultiplemetabolicpathways.

UC是一种慢性难治性非特异性IBD。新UC相关代谢生物标志物的发现可能有助于进一步了解UC和早期诊断，同时也可以为解释UC治疗中药物作用机理提供基础。复方苦参汤（CSD）是一个临床治疗UC的验方，但尚不清楚其在UC治疗中的作用机制。这项研究的目的是通过代谢组学方法研究UC大鼠内源性物质的变化以及CSD对代谢途径的影响。使用LC-MS/MS在UC大鼠和正常大鼠中进行代谢组学研究。使用TNBS灌肠诱导的UC大鼠作为研究模型。通过相关统计分析进行生物标志物的代谢谱分析和途径分析。UC组和正常组之间有36种潜在的生物标志物存在显著差异。研究还发现，CSD可以调节这些潜在生物标志物水平。我们通过调节多种代谢途径，发现CSD在UC中是有效的。